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A genetic assay for gene essentiality in Clostridium
File(s)
DWAnaerobe.pdf (1.41 MB)
Accepted version
Author(s)
Walker, DJ
Heap, JT
Winzer, K
Minton, NP
Type
Journal Article
Abstract
Essential genes of pathogens are potential therapeutic targets, but are difficult to verify. Here, gene essentiality was determined by targeted knockout following engineered gene duplication. Null mutants of candidate essential genes of Clostridium difficile were viable only in the presence of a stable second copy of the gene.
Date Issued
2016-07-31
Date Acceptance
2016-07-29
Citation
Anaerobe, 2016, 42, pp.40-43
URI
DOI
ISSN
1095-8274
Publisher
Elsevier
Start Page
40
End Page
43
Journal / Book Title
Anaerobe
Volume
42
Copyright Statement
Creative Commons Attribution 4.0 International (CC BY 4.0)
License URL
Subjects
ClosTron
Clostridium difficile
Gene essentiality
Merodiploid
Therapeutic target
Microbiology
0605 Microbiology
1108 Medical Microbiology
Publication Status
Published