Characterization of neutrophil subsets in healthy human pregnancies
File(s)
Author(s)
Type
Journal Article
Abstract
We have previously shown that in successful pregnancies increased arginase activity is a mechanism that contributes to the suppression of the maternal immune system. We identified the main type of arginase-expressing cells as a population of activated low-density granulocytes (LDGs) in peripheral blood mononuclear cells and in term placentae. In the present study, we analyzed the phenotype of LDGs and compared it to the phenotype of normal density granulocytes (NDGs) in maternal peripheral blood, placental biopsies and cord blood. Our data reveal that only LDGs but no NDGs could be detected in placental biopsies. Phenotypically, NDGs and LDGs from both maternal and cord blood expressed different levels of maturation, activation and degranulation markers. NDGs from the maternal and cord blood were phenotypically similar, while maternal, cord and placental LDGs showed different expression levels of CD66b. LDGs present in cord blood expressed higher levels of arginase compared to maternal and placental LDGs. In summary, our results show that in maternal and cord blood, two phenotypically different populations of neutrophils can be identified, whereas in term placentae, only activated neutrophils are present.
Date Issued
2014-02-13
Date Acceptance
2013-12-06
Citation
PLOS One, 2014, 9 (2)
ISSN
1932-6203
Publisher
Public Library of Science
Journal / Book Title
PLOS One
Volume
9
Issue
2
Copyright Statement
© 2014 Ssemaganda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
License URL
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MULTIDISCIPLINARY SCIENCES
THERAPEUTIC PERSPECTIVES
ARGINASE ACTIVITY
SUPPRESSOR-CELLS
OXIDANT RELEASE
IMMUNE-SYSTEM
L-ARGININE
METABOLISM
GRANULOCYTES
MECHANISMS
EXPRESSION
Adult
Antigens, CD
Arginase
Biomarkers
Cell Adhesion Molecules
Cell Degranulation
Cell Lineage
Female
Fetal Blood
GPI-Linked Proteins
Gene Expression
Humans
Immune Tolerance
Immunophenotyping
Leukocyte Count
Neutrophil Activation
Neutrophils
Phenotype
Placenta
Pregnancy
Biological Markers
General Science & Technology
MD Multidisciplinary
Publication Status
Published
Article Number
e85696