Single-molecule RNA Imaging Using Mango II Arrays
Author(s)
Rueda, David
Cawte, Adam D
Ino, Haruki
Unrau, Peter
Type
Chapter
Abstract
In recent years, fluorogenic RNA aptamers, such as Spinach, Broccoli, Corn, Mango, Coral, and Pepper have gathered traction as an efficient alternative labeling strategy for background-free imaging of cellular RNAs. However, their application has been somewhat limited by relatively inefficient folding and fluorescent stability. With the recent advent of novel RNA-Mango variants which are improved in both fluorescence intensity and folding stability in tandem arrays, it is now possible to image RNAs with single-molecule sensitivity. Here we discuss the protocol for imaging Mango II tagged RNAs in both fixed and live cells.
Date Issued
2021-10-26
Citation
Methods in Molecular Biology, 2021
Publisher
Humana Press
Journal / Book Title
Methods in Molecular Biology
Copyright Statement
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Sponsor
Medical Research Council
Identifier
https://link.springer.com/protocol/10.1007%2F978-1-0716-1851-6_14
Grant Number
MC_UP_1102/5
Subjects
Fluorogenic RNA Aptamer
Live cell imaging
RNA dynamics
RNA-Mango
Single-Molecule Microscopy
Superresolution microscopy
Aptamers, Nucleotide
Fluorescent Dyes
Mangifera
RNA
Spinacia oleracea
0399 Other Chemical Sciences
0601 Biochemistry and Cell Biology
Developmental Biology
Publication Status
Published
Date Publish Online
2021-10-26