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Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold
File(s)
OA Location
Author(s)
Jia, Y
Benjamin, S
Liu, Q
Xu, Y
Dogga, SK
Type
Journal Article
Abstract
The immune mapped protein 1 (IMP1) was first identified as a protective antigen in Eimeria maxima and described as vaccine candidate and invasion factor in Toxoplasma gondii. We show here that TgIMP1 localizes to the inner leaflet of plasma membrane (PM) via dual acylation. Mutations either in the N-terminal myristoylation or palmitoylation sites (G2 and C5) cause relocalization of TgIMP1 to the cytosol. The first 11 amino acids are sufficient for PM targeting and the presence of lysine (K7) is critical. Disruption of TgIMP1 gene by double homologous recombination revealed no invasion defect or any measurable alteration in the lytic cycle of tachyzoites. Following immunization with TgIMP1 DNA vaccine, mice challenged with either wild type or IMP1-ko parasites showed no significant difference in protection. The sequence analysis identified a structured C-terminal domain that is present in a broader family of IMP1-like proteins conserved across the members of Apicomplexa. We present the solution structure of this domain determined from NMR data and describe a new protein fold not seen before.
Date Issued
2016-11-22
Date Acceptance
2016-11-21
Citation
Biochimica et Biophysica Acta - Proteins and Proteomics, 2016, 1865 (2), pp.208-219
URI
DOI
ISSN
1570-9639
Publisher
Elsevier
Start Page
208
End Page
219
Journal / Book Title
Biochimica et Biophysica Acta - Proteins and Proteomics
Volume
1865
Issue
2
Copyright Statement
© 2016, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Sponsor
Medical Research Council (MRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Medical Research Council (MRC)
Grant Number
G0400423
BB/E02520X/1
G0800038
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biophysics
Toxoplasma gondii
Acylation
Invasion
Vaccine
NMR
Protein structure
PALMITOYLATED PROTEINS
GLOBAL ANALYSIS
HIGH-THROUGHPUT
ASSIGNMENT
PARASITES
DOMAINS
IDENTIFICATION
EXPRESSION
ALIGNMENT
NEOSPORA
Publication Status
Published