Pseudomonas aeruginosa adaptation and diversification in the non-cystic fibrosis bronchiectasis lung
Author(s)
Type
Journal Article
Abstract
To characterise Pseudomonas aeruginosa populations during chronic lung infections of non-cystic fibrosis bronchiectasis patients, we used whole-genome sequencing to 1) assess the diversity of P. aeruginosa and the prevalence of multilineage infections; 2) seek evidence for cross-infection or common source acquisition; and 3) characterise P. aeruginosa adaptations.
189 isolates, obtained from the sputa of 91 patients attending 16 adult bronchiectasis centres in the UK, were whole-genome sequenced.
Bronchiectasis isolates were representative of the wider P. aeruginosa population. Of 24 patients from whom multiple isolates were examined, there were seven examples of multilineage infections, probably arising from multiple infection events. The number of nucleotide variants between genomes of isolates from different patients was in some cases similar to the variations observed between isolates from individual patients, implying the possible occurrence of cross-infection or common source acquisition.
Our data indicate that during infections of bronchiectasis patients, P. aeruginosa populations adapt by accumulating loss-of-function mutations, leading to changes in phenotypes including different modes of iron acquisition and variations in biofilm-associated polysaccharides. The within-population diversification suggests that larger scale longitudinal surveillance studies will be required to capture cross-infection or common source acquisition events at an early stage.
189 isolates, obtained from the sputa of 91 patients attending 16 adult bronchiectasis centres in the UK, were whole-genome sequenced.
Bronchiectasis isolates were representative of the wider P. aeruginosa population. Of 24 patients from whom multiple isolates were examined, there were seven examples of multilineage infections, probably arising from multiple infection events. The number of nucleotide variants between genomes of isolates from different patients was in some cases similar to the variations observed between isolates from individual patients, implying the possible occurrence of cross-infection or common source acquisition.
Our data indicate that during infections of bronchiectasis patients, P. aeruginosa populations adapt by accumulating loss-of-function mutations, leading to changes in phenotypes including different modes of iron acquisition and variations in biofilm-associated polysaccharides. The within-population diversification suggests that larger scale longitudinal surveillance studies will be required to capture cross-infection or common source acquisition events at an early stage.
Date Issued
2017-04-01
Date Acceptance
2017-01-21
Citation
European Respiratory Journal, 2017, 49 (4), pp.1-8
ISSN
0903-1936
Publisher
European Respiratory Society
Start Page
1
End Page
8
Journal / Book Title
European Respiratory Journal
Volume
49
Issue
4
Copyright Statement
©ERS 2017. This ERJ Open article is open access and distributed under the terms of the Creative Commons
Attribution Licence 4.0.
Attribution Licence 4.0.
License URL
Sponsor
National Institute for Health Research
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000400342500022&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
BRU 6279
Subjects
Science & Technology
Life Sciences & Biomedicine
Respiratory System
DI-GMP PHOSPHODIESTERASE
BIOFILM FORMATION
INFECTION
POPULATION
RESISTANCE
DIVERSITY
SECRETION
PATHOGEN
SEQUENCE
STRAINS
Publication Status
Published
Article Number
ARTN 1602108
Date Publish Online
2017-04-26