Cooperation between interleukin-5 and the chemokine eotaxin to induce eosinophil accumulation in vivo.
Author(s)
Collins, PD
Marleau, S
Griffiths-Johnson, DA
Jose, PJ
Williams, TJ
Type
Journal Article
Abstract
Experiments were designed to study the effect of systemically administered IL-5 on local eosinophil accumulation induced by the intradermal injection of the chemokine eotaxin in the guinea pig. Intravenous interleukin-5 (IL-5) stimulated a rapid and dramatic increase in the numbers of accumulating eosinophils induced by i.d.-injected eotaxin and, for comparison, leukotriene B4. The numbers of locally accumulating eosinophils correlated directly with a rapid increase in circulating eosinophils: circulating eosinophil numbers were 13-fold higher 1 h after intravenous IL-5 (18.3 pmol/kg). This increase in circulating cells corresponded with a reduction in the number of displaceable eosinophils recovered after flushing out the femur bone marrow cavity. Intradermal IL-5, at the doses tested, did not induce significant eosinophil accumulation. We propose that these experiments simulate important early features of the tissue response to local allergen exposure in a sensitized individual, with eosinophil chemoattractant chemokines having an important local role in eosinophil recruitment from blood microvessels, and IL-5 facilitating this process by acting remotely as a hormone to stimulate the release into the circulation of a rapidly mobilizable pool of bone marrow eosinophils. This action of IL-5 would be complementary to the other established activities of IL-5 that operate over a longer time course.
Date Issued
1995-10-01
Date Acceptance
1995-01-01
Citation
Journal of Experimental Medicine, 1995, 182 (4), pp.1169-1174
ISSN
1540-9538
Publisher
Rockefeller University Press
Start Page
1169
End Page
1174
Journal / Book Title
Journal of Experimental Medicine
Volume
182
Issue
4
Copyright Statement
© The Rockefeller University Pres
Subjects
Animals
Bone Marrow
Bone Marrow Cells
Chemokine CCL11
Chemokines, CC
Chemotaxis, Leukocyte
Cytokines
Dose-Response Relationship, Drug
Drug Synergism
Eosinophil Peroxidase
Eosinophilia
Eosinophils
Guinea Pigs
Injections, Intradermal
Injections, Intravenous
Interleukin-5
Leukotriene B4
Male
Peroxidases
Skin
Publication Status
Published