Imaging tumor microscopic viscosity in vivo using molecular rotors
File(s)
Supporting information
Published version
Author(s)
Type
Journal Article
Abstract
The microscopic viscosity plays an essential role in cellular biophysics by controlling the rates of diffusion and bimolecular reactions within the cell interior. While several approaches have emerged that have allowed the measurement of viscosity and diffusion on a single cell level in vitro, the in vivo viscosity monitoring has not yet been realized. Here we report the use of fluorescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image microscopic viscosity in vivo, both on a single cell level and in connecting tissues of subcutaneous tumors in mice. We find that viscosities recorded from single tumor cells in vivo correlate well with the in vitro values from the same cancer cell line. Importantly, our new method allows both imaging and dynamic monitoring of viscosity changes in real time in live animals and thus it is particularly suitable for diagnostics and monitoring of the progress of treatments that might be accompanied by changes in microscopic viscosity.
Date Issued
2017-01-30
Date Acceptance
2016-12-12
Citation
Scientific Reports, 2017, 7
ISSN
2045-2322
Publisher
Nature Publishing Group
Journal / Book Title
Scientific Reports
Volume
7
Copyright Statement
© 2017 The Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
License URL
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Commission of the European Communities
The Royal Society
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000392849100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
EP/I003983/1
327334
UF140161
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
FLUORESCENCE CORRELATION SPECTROSCOPY
LIVE CELLS
PHOTODYNAMIC THERAPY
CELLULAR-VISCOSITY
PLASMA-MEMBRANES
PHASE-SEPARATION
INNER MEMBRANE
ACID) BRUSHES
LIVING CELLS
SINGLE-CELL
Publication Status
Published
Article Number
ARTN 41097