Fiber-based electrochemical biosensors for monitoring pH and transient neurometabolic lactate.
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Developing tools that are able to monitor transient neurochemical dynamics is important to decipher brain chemistry and function. Multifunctional polymer-based fibers have been recently applied to monitor and modulate neural activity. Here, we explore the potential of polymer fibers comprising six graphite-doped electrodes and two microfluidic channels within a flexible polycarbonate body as a platform for sensing pH and neurometabolic lactate. Electrodes were made into potentiometric sensors (responsive to pH) or amperometric sensors (lactate biosensors). The growth of an iridium oxide layer made the fiber electrodes responsive to pH in a physiologically relevant range. Lactate biosensors were fabricated via platinum black growth on the fiber electrode, followed by an enzyme layer, making them responsive to lactate concentration. Lactate fiber biosensors detected transient neurometabolic lactate changes in an in vivo mouse model. Lactate concentration changes were associated with spreading depolarizations, known to be detrimental to the injured brain. Induced waves were identified by a signature lactate concentration change profile and measured as having a speed of ∼2.7 mm/min (n = 4 waves). Our work highlights the potential applications of fiber-based biosensors for direct monitoring of brain metabolites in the context of injury.
Date Issued
2021-05-04
Date Acceptance
2021-03-22
Citation
Analytical Chemistry, 2021, 93 (17), pp.6646-6655
ISSN
0003-2700
Publisher
American Chemical Society
Start Page
6646
End Page
6655
Journal / Book Title
Analytical Chemistry
Volume
93
Issue
17
Copyright Statement
© 2021 The Authors. Published by American Chemical Society. This article is published under CC BY 4.0 International license.
License URL
Sponsor
Medical Research Council (MRC)
Engineering & Physical Science Research Council (EPSRC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/33797893
Grant Number
MR/R015651/1
EP/K020641/1
Subjects
Analytical Chemistry
0301 Analytical Chemistry
0399 Other Chemical Sciences
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2021-04-02