Replicating landmine blast loading in cellular <i>in Vitro</i> models
File(s)
Published version
Supporting information
Author(s)
Sory, David R
Amin, Harsh D
Chapman, David
Proud, WG
Rankin, Sara M
Type
Journal Article
Abstract
Trauma arising from landmines and improvised explosive devices promotes heterotopic ossification, the formation of extra-skeletal bone in non-osseous tissue. To date, experimental platforms that can replicate the loading parameter space relevant to improvised explosive device and landmine blast wave exposure have not been available to study the effects of such non-physiological mechanical loading on cells. Here, we present the design and calibration of three distinct in vitro experimental loading platforms that allow us to replicate the spectrum of loading conditions recorded in near-field blast wave exposure. We subjected cells in suspension or in a three-dimensional hydrogel to strain rates up to 6000 s-1and pressure levels up to 45 MPa. Our results highlight that cellular activation is regulated in a non-linear fashion - not by a single mechanical parameter, it is the combined action of the applied mechanical pressure, rate of loading and loading impulse, along with the extracellular environment used to convey the pressure waves. Finally, our research indicates that PO MSCs are finely tuned to respond to mechanical stimuli that fall within defined ranges of loading.
Date Issued
2020-09-01
Date Acceptance
2020-03-05
Citation
Physical Biology, 2020, 17 (5)
ISSN
1478-3967
Publisher
IOP Publishing
Journal / Book Title
Physical Biology
Volume
17
Issue
5
Copyright Statement
s © 2020 The Author(s). Published by IOP Publishing Ltd. As the Version of Record of this article is going to be / has been published on a gold open access basis under a CC BY 3.0 licence, this Accepted
Manuscript is available for reuse under a CC BY 3.0 licence immediately.
Everyone is permitted to use all or part of the original content in this article, provided that they adhere to all the terms of the licence
https://creativecommons.org/licences/by/3.0
Manuscript is available for reuse under a CC BY 3.0 licence immediately.
Everyone is permitted to use all or part of the original content in this article, provided that they adhere to all the terms of the licence
https://creativecommons.org/licences/by/3.0
Sponsor
The Royal British Legion
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/32141440
Grant Number
BMPF_P60304
Subjects
anti personnel landmine
blast injuries
osteogenesis
periosteum
split-Hopkinson pressure bar
stem cell
strain rate
Publication Status
Published
Coverage Spatial
England
Date Publish Online
2020-03-05