Rapid and self-administrable capillary blood microsampling demonstrates statistical equivalence with standard venous collections in NMR-based lipoprotein analysis
Author(s)
Type
Journal Article
Abstract
We investigated plasma and serum blood derivatives from capillary blood microsamples (500 μL, MiniCollect tubes) and corresponding venous blood (10 mL vacutainers). Samples from 20 healthy participants were analyzed by 1H NMR, and 112 lipoprotein subfraction parameters; 3 supramolecular phospholipid composite (SPC) parameters from SPC1, SPC2, and SPC3 subfractions; 2 N-acetyl signals from α-1-acid glycoprotein (Glyc), GlycA, and GlycB; and 3 calculated parameters, SPC (total), SPC3/SPC2, and Glyc (total) were assessed. Using linear regression between capillary and venous collection sites, we explained that agreement (Adj. R2 ≥ 0.8, p < 0.001) was witnessed for 86% of plasma parameters (103/120) and 88% of serum parameters (106/120), indicating that capillary lipoprotein, SPC, and Glyc concentrations follow changes in venous concentrations. These results indicate that capillary blood microsamples are suitable for sampling in remote areas and for high-frequency longitudinal sampling of the majority of lipoproteins, SPCs, and Glycs.
Date Issued
2024-02-19
Date Acceptance
2024-01-29
Citation
Analytical Chemistry, 2024, 96 (11), pp.4505-4513
ISSN
0003-2700
Publisher
American Chemical Society
Start Page
4505
End Page
4513
Journal / Book Title
Analytical Chemistry
Volume
96
Issue
11
Copyright Statement
Copyright © 2024 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY-NC-ND 4.0 .
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/38372289
Subjects
ASSIGNMENT
BIOMARKER
Chemistry
Chemistry, Analytical
H-1
H-1-NMR
HEALTH
NUCLEAR-MAGNETIC-RESONANCE
Physical Sciences
PLASMA-LIPOPROTEINS
Science & Technology
SERUM
SPECTROSCOPY
URINE
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2024-02-19