Biomethanation and microbial community changes in a digester treating sludge from a brackish aquaculture recirculation system
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Using a high-salinity-adapted inoculum and a moderate stepwise-increased organic loading rate (OLR), a stable digester performance was achieved in treating sludge from a brackish aquaculture recirculation system. The specific methane yield was distinctly enhanced, reaching 0.203 L CH4/g CODadded, compared to literature values (0.140–0.154 L CH4/g CODadded) from the salty sludges. OLR adjustment and the fecal substrate substantially influenced population changes in the digester. Within the bacterial subpopulations, the relative abundance of Bacillus and Bacteroides declined, accompanied by the increase of Clostridium and Trigonala over time. The results show Trigonala was derived from the substrate and accumulated inside the digester. The most abundant methanogen was Methanosarcina in the inoculum and the digestates. The Methanosarcina proliferation can be ascribed to its metabolic versatility, probably a feature of crucial importance for high-salinity environments. Other frequently observed methanogens were outcompeted. The population similarity at the genus level between inoculum and digestates declined during the initial stage and afterwards increased.
Date Issued
2016-04-28
Date Acceptance
2016-04-21
Citation
Bioresource Technology, 2016, 214, pp.338-347
ISSN
1873-2976
Publisher
Elsevier
Start Page
338
End Page
347
Journal / Book Title
Bioresource Technology
Volume
214
Copyright Statement
© 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CCBY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Subjects
Science & Technology
Life Sciences & Biomedicine
Technology
Agricultural Engineering
Biotechnology & Applied Microbiology
Energy & Fuels
Agriculture
Anaerobic digestion
Brackish aquaculture recirculation systems
Stepwise increased OLR
Sludge retention time (SRT)
Inoculum
Bacterial and archaeal population
MESOPHILIC ANAEROBIC TREATMENT
RETENTION TIME
CO-DIGESTION
SOLID-WASTE
BACTERIAL
VERRUCOMICROBIA
INHIBITION
AMMONIA
REACTOR
MANURE
Ammonium Compounds
Aquaculture
Archaea
Bacteria
Batch Cell Culture Techniques
Biodegradation, Environmental
Biodiversity
Biofuels
Bioreactors
Hydrogen-Ion Concentration
Methane
Phylogeny
Salinity
Sewage
Biotechnology
MD Multidisciplinary
Publication Status
Published