Effects of bovine serum albumin on light activated antimicrobial surfaces
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Bovine serum albumin (BSA) is currently recommended as an interfering substance to emulate organic soiling, in evaluating the efficacy of disinfectants. The European Standard recommends 0.03% BSA to test clean conditions and 0.3% for dirty conditions. Reactive oxygen species are known to exert excellent antimicrobial activity with low specificity against a broad range of pathogens. Herein, we present our data from the first study of the effects of the addition of BSA on the antibacterial activity of light activated antimicrobial surfaces. Light activated antimicrobial surfaces were made from polyurethane swell-encapsulated with gold nanoparticles (AuNPs) coated with the light active triarylmethane dye, crystal violet (PU-AuNP-CV). The antibacterial efficacy of the antimicrobial substrates was tested against two strains of Staphylococcus aureus 8325-4, a well-characterised laboratory strain and MRSA 4742, a recent clinical isolate, in the presence of 0.1% to 1% BSA by irradiating the substrates with a fluorescent lamp (300 lux). After 6 hours of irradiation, the number of surviving bacteria was determined. The results showed that BSA reduced the antibacterial efficacy of all the PU-AuNP-CV surfaces with increasing BSA concentrations resulting in a progressive reduction in antibacterial activity towards the bacteria tested. However, the light activated surfaces did perform well at 0.1 and 0.25% BSA levels, showing they may have potential for real world environments with low levels of organic soiling.
Date Issued
2018-10-05
Date Acceptance
2018-09-12
Citation
RSC Advances: an international journal to further the chemical sciences, 2018, 8 (60), pp.34252-34258
ISSN
2046-2069
Publisher
Royal Society of Chemistry
Start Page
34252
End Page
34258
Journal / Book Title
RSC Advances: an international journal to further the chemical sciences
Volume
8
Issue
60
Copyright Statement
© The Royal Society of Chemistry 2018. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (http://creativecommons.org/licenses/by/3.0/).
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000448423500008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
GRAM-NEGATIVE BACTERIA
STAPHYLOCOCCUS-AUREUS
GOLD NANOPARTICLES
ESCHERICHIA-COLI
ANTIBACTERIAL ACTIVITY
HYDROGEN-PEROXIDE
REACTIVE OXYGEN
RESISTANCE
TITANIA
POLYURETHANE
Publication Status
Published
Date Publish Online
2018-10-05