Effect of irradiation/bone marrow transplantation on alveolar epithelial type II cells is aggravated in surfactant protein D deficient mice.
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Irradiation followed by bone marrow transplantation (BM-Tx) is a frequent therapeutic intervention causing pathology to the lung. Although alveolar epithelial type II (AE2) cells are essential for lung function and are damaged by irradiation, the long-term consequences of irradiation and BM-Tx are not well characterized. In addition, it is unknown whether surfactant protein D (SP-D) influences the response of AE2 cells to the injurious events. Therefore, wildtype (WT) and SP-D(-/-) mice were subjected to a myeloablative whole body irradiation dose of 8 Gy and subsequent BM-Tx and compared with age- and sex-matched untreated controls. AE2 cell changes were investigated quantitatively by design-based stereology. Compared with WT, untreated SP-D(-/-) mice showed a higher number of larger sized AE2 cells and a greater amount of surfactant-storing lamellar bodies. Irradiation and BM-Tx induced hyperplasia and hypertrophy in WT and SP-D(-/-) mice as well as the formation of giant lamellar bodies. The experimentally induced alterations were more severe in the SP-D(-/-) than in the WT mice, particularly with respect to the surfactant-storing lamellar bodies which were sometimes extremely enlarged in SP-D(-/-) mice. In conclusion, irradiation and BM-Tx have profound long-term effects on AE2 cells and their lamellar bodies. These data may explain some of the clinical pulmonary consequences of this procedure. The data should also be taken into account when BM-Tx is used as an experimental procedure to investigate the impact of bone marrow-derived cells for the phenotype of a specific genotype in the mouse.
Date Issued
2016-08-26
Date Acceptance
2016-08-16
Citation
Histochemistry and Cell Biology, 2016, 147 (1), pp.49-61
ISSN
0948-6143
Publisher
Springer Verlag
Start Page
49
End Page
61
Journal / Book Title
Histochemistry and Cell Biology
Volume
147
Issue
1
Copyright Statement
© 2016 Springer-Verlag. The final publication is available at Springer via https://dx.doi.org/10.1007/s00418-016-1479-7
Subjects
Science & Technology
Life Sciences & Biomedicine
Technology
Cell Biology
Microscopy
Irradiation
Bone marrow transplantation
Design-based stereology
Alveolar epithelium
Surfactant
COLONY-STIMULATING FACTOR
PULMONARY HOST-DEFENSE
TOTAL-BODY IRRADIATION
INDUCED LUNG INJURY
BONE-MARROW
MOUSE LUNG
STEREOLOGICAL ANALYSIS
RADIATION PNEUMONITIS
LATE-PHASE
MODEL
Alveolar Epithelial Cells
Animals
Bone Marrow Transplantation
Gamma Rays
Mice
Mice, Inbred C57BL
Mice, Knockout
Pulmonary Surfactant-Associated Protein D
Whole-Body Irradiation
1199 Other Medical And Health Sciences
Biochemistry & Molecular Biology
Publication Status
Published