Model of host-pathogen Interaction dynamics links In vivo optical imaging and immune responses
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Tracking disease progression in vivo is essential for the development of treatments against bacterial infection. Optical imaging has become a central tool for in vivo tracking of bacterial population development and therapeutic response. For a precise understanding of in vivo imaging results in terms of disease mechanisms derived from detailed postmortem observations, however, a link between the two is needed. Here, we develop a model that provides that link for the investigation of Citrobacter rodentium infection, a mouse model for enteropathogenic Escherichia coli (EPEC). We connect in vivo disease progression of C57BL/6 mice infected with bioluminescent bacteria, imaged using optical tomography and X-ray computed tomography, to postmortem measurements of colonic immune cell infiltration. We use the model to explore changes to both the host immune response and the bacteria and to evaluate the response to antibiotic treatment. The developed model serves as a novel tool for the identification and development of new therapeutic interventions.
Date Issued
2016-11-07
Date Acceptance
2016-10-16
Citation
Infection and Immunity, 2016, 85 (1)
ISSN
1098-5522
Publisher
American Society for Microbiology
Journal / Book Title
Infection and Immunity
Volume
85
Issue
1
Copyright Statement
© 2016 The Authors.This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/).
License URL
Sponsor
The Royal Society
Medical Research Council (MRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
Identifier
http://www.ncbi.nlm.nih.gov/pubmed/27821583
PII: IAI.00606-16
Grant Number
WRMA09R3/HLL
MR/K022040/1
BB/K017284/1
BB/N011597/1
Subjects
Citrobacter rodentium
antibiotic therapy
bioluminescent bacteria
dynamic modeling
espO mutant
in vivo imaging
Microbiology
06 Biological Sciences
11 Medical And Health Sciences
07 Agricultural And Veterinary Sciences
Publication Status
Published
Coverage Spatial
United States
Article Number
e00606-16