Predicting translation initiation rates for designing synthetic biology.
File(s)
Author(s)
Reeve, Benjamin
Hargest, Thomas
Gilbert, Charlie
Ellis, Tom
Type
Journal Article
Abstract
In synthetic biology, precise control over protein expression is required in order to construct functional biological systems. A core principle of the synthetic biology approach is a model-guided design and based on the biological understanding of the process, models of prokaryotic protein production have been described. Translation initiation rate is a rate-limiting step in protein production from mRNA and is dependent on the sequence of the 5'-untranslated region and the start of the coding sequence. Translation rate calculators are programs that estimate protein translation rates based on the sequence of these regions of an mRNA, and as protein expression is proportional to the rate of translation initiation, such calculators have been shown to give good approximations of protein expression levels. In this review, three currently available translation rate calculators developed for synthetic biology are considered, with limitations and possible future progress discussed.
Date Issued
2014-01-20
Date Acceptance
2014-01-06
Citation
Frontiers in Bioengineering and Biotechnology, 2014, 2, pp.1-1
ISSN
2296-4185
Publisher
Frontiers Media
Start Page
1
End Page
1
Journal / Book Title
Frontiers in Bioengineering and Biotechnology
Volume
2
Copyright Statement
Copyright: © 2014 Reeve, Hargest, Gilbert and Ellis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
License URL
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/25152877
Grant Number
EP/G036004/1
Subjects
5′-untranslated region
RBS Calculator
RBS Designer
UTR Designer
ribosome binding site
synthetic biology
translation efficiency
translation rate
Publication Status
Published online
Coverage Spatial
Switzerland