Microscopic viscosity of neuronal plasma membranes measured using fluorescent molecular rotors: effects of oxidative stress and neuroprotection.
File(s)
Accepted version
Supporting information
Author(s)
Kubankova, Marketa
Summers, Peter
Lopez-Duarte, Ismael
Kiryushko, Darya
Kuimova, Marina K
Type
Journal Article
Abstract
Molecular mobility in neuronal plasma membranes is a crucial factor in brain function. Microscopic viscosity is an important parameter that determines molecular mobility. This study presents the first direct measurements of the microviscosity of plasma membranes of live neurons. Microviscosity maps were obtained using fluorescence lifetime imaging of environment-sensing dyes termed 'molecular rotors'. Neurons were investigated both in the basal state and following common neurodegenerative stimuli, excitotoxicity or oxidative stress. Both types of neurotoxic challenges induced microviscosity decrease in cultured neurons, and the oxidant-induced membrane fluidification was counteracted by the wide-spectrum neuroprotectant, the H3 peptide. These results provide new insights into molecular mobility in neuronal membranes, paramount for basic brain function, and suggest that preservation of membrane stability may be an important aspect of neuroprotection in brain insults and neurodegenerative disorders.
Date Issued
2019-10-09
Date Acceptance
2019-09-12
Citation
ACS Applied Materials and Interfaces, 2019, 11 (40), pp.36307-36315
ISSN
1944-8244
Publisher
American Chemical Society
Start Page
36307
End Page
36315
Journal / Book Title
ACS Applied Materials and Interfaces
Volume
11
Issue
40
Copyright Statement
© 2019 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsami.9b10426
Sponsor
Commission of the European Communities
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Engineering & Physical Science Research Council (EPSRC)
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/31513373
Grant Number
PIEF-GA-2013-627049
EP/I003983/1
EP/K030760/1
n/a
Subjects
H3 peptide
excitotoxicity
fluorescence lifetime imaging
microviscosity
molecular rotor
neurons
neuroprotection
oxidative stress
plasma membrane
0904 Chemical Engineering
0303 Macromolecular and Materials Chemistry
0306 Physical Chemistry (incl. Structural)
Nanoscience & Nanotechnology
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2019-09-12