miR-17-5p regulates endocytic trafficking through targeting TBC1D2/ Armus
File(s)
Author(s)
Type
Journal Article
Abstract
miRNA cluster miR-17-92 is known as oncomir-1 due to its potent oncogenic function. miR-17-92 is a polycistronic cluster
that encodes 6 miRNAs, and can both facilitate and inhibit cell proliferation. Known targets of miRNAs encoded by this
cluster are largely regulators of cell cycle progression and apoptosis. Here, we show that miRNAs encoded by this cluster
and sharing the seed sequence of miR-17 exert their influence on one of the most essential cellular processes – endocytic
trafficking. By mRNA expression analysis we identified that regulation of endocytic trafficking by miR-17 can potentially be
achieved by targeting of a number of trafficking regulators. We have thoroughly validated TBC1D2/Armus, a GAP of Rab7
GTPase, as a novel target of miR-17. Our study reveals regulation of endocytic trafficking as a novel function of miR-17,
which might act cooperatively with other functions of miR-17 and related miRNAs in health and disease.
that encodes 6 miRNAs, and can both facilitate and inhibit cell proliferation. Known targets of miRNAs encoded by this
cluster are largely regulators of cell cycle progression and apoptosis. Here, we show that miRNAs encoded by this cluster
and sharing the seed sequence of miR-17 exert their influence on one of the most essential cellular processes – endocytic
trafficking. By mRNA expression analysis we identified that regulation of endocytic trafficking by miR-17 can potentially be
achieved by targeting of a number of trafficking regulators. We have thoroughly validated TBC1D2/Armus, a GAP of Rab7
GTPase, as a novel target of miR-17. Our study reveals regulation of endocytic trafficking as a novel function of miR-17,
which might act cooperatively with other functions of miR-17 and related miRNAs in health and disease.
Date Issued
2012-12-20
Date Acceptance
2012-11-15
Citation
PLoS ONE, 2012, 7
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Journal / Book Title
PLoS ONE
Volume
7
Copyright Statement
©
2012 Serva et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
2012 Serva et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Sponsor
Medical Research Council (MRC)
Cancer Research UK
Grant Number
G0600791
C1282/A11980
Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MULTIDISCIPLINARY SCIENCES
LOW-DENSITY-LIPOPROTEIN
CANCER CELL-PROLIFERATION
EPIDERMAL-GROWTH-FACTOR
HAMSTER OVARY CELLS
E-CADHERIN
BREAST-CANCER
ENDOSOMAL TRAFFICKING
MICRORNA CLUSTER
DOWN-REGULATION
MESSENGER-RNAS
Base Sequence
Cell Proliferation
Down-Regulation
Endocytosis
Epidermal Growth Factor
GTPase-Activating Proteins
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
HeLa Cells
Humans
MicroRNAs
Molecular Sequence Data
Protein Structure, Tertiary
Protein Transport
RNA, Messenger
Receptors, LDL
Reproducibility of Results
Hela Cells
MD Multidisciplinary
General Science & Technology
Publication Status
Published
Article Number
e52555