Developing a food exposure and urine sampling strategy for dietary exposure biomarker validation in free-living individuals
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
SCOPE: Dietary choices modulate the risk of chronic diseases and improving diet is a central component of public health strategies. Food-derived metabolites present in urine could provide objective biomarkers of dietary exposure. To assist biomarker validation we aimed to develop a food intervention strategy mimicking a typical annual diet over a short period of time and assessed urine sampling protocols potentially suitable for future deployment of biomarker technology in free-living populations. METHODS AND RESULTS: Six different menu plans representing comprehensively a typical UK annual diet that were split into two dietary experimental periods. Free-living adult participants (n = 15 and n = 36, respectively) were provided with all their food, as a series of menu plans, over a period of 3 consecutive days. Multiple spot urine samples were collected and stored at home. CONCLUSION: We established a successful food exposure strategy following a conventional UK eating pattern, which was suitable for biomarker validation in free-living individuals. The urine sampling procedure was acceptable for volunteers and delivered samples suitable for biomarker quantification. Our study design provides scope for validation of existing biomarker candidates and potentially for discovery of new biomarker-leads and should help inform the future deployment of biomarker technology for habitual dietary exposure measurement.
Date Issued
2019-07
Date Acceptance
2019-06-01
Citation
Molecular Nutrition and Food Research, 2019, 63 (14), pp.1-9
ISSN
1613-4125
Publisher
Wiley
Start Page
1
End Page
9
Journal / Book Title
Molecular Nutrition and Food Research
Volume
63
Issue
14
Copyright Statement
©2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co.KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distributionand reproduction in any medium, provided the original work is properlycited.
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/31157514
Subjects
Dietary-exposure
biomarker
free-living
metabolomics
urine-sampling
Publication Status
Published
Coverage Spatial
Germany
Article Number
1900062
Date Publish Online
2019-06-17