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Simple and cost-effective cross-polarization experiments under dissolution-dynamic nuclear polarization conditions with a 3D-printed ¹H-¹³C background-free radiofrequency coil
File(s)
Author(s)
Elliott, Stuart J
Ceillier, Morgan
Cala, Olivier
Stern, Quentin
Cousin, Samuel F
Type
Journal Article
Abstract
The low sensitivity of conventional nuclear magnetic resonance experiments can be overcome, in suitable cases, by employing hyperpolarization methodologies. One such technique, dissolution-dynamic nuclear polarization, provides a robust means of strongly polarizing a variety of small molecules. A drawback of the dissolution-dynamic nuclear polarization approach, the excessively long polarization timescales for insensitive nuclei, has been circumvented by using cross-polarization radiofrequency pulse sequences, which in general yield quick and substantial 13C polarizations. However, the capacity to effectively perform efficient cross-polarization experiments under dissolution-dynamic nuclear polarization conditions remains challenging, and polarization quantification can be plagued by additional complications including spurious background signals from the probe. Here we propose a background-free 1H-13C radiofrequency coil specifically designed for use in cross-polarization experiments on samples of up to 500 μL in volume at liquid helium temperatures. We additionally introduce simple guidelines for the optimization and implementation of cross-polarization radiofrequency pulse sequences. Experimental demonstrations of 13C polarizations reaching ∼60% are presented for the case of [1-13C]sodium acetate.
Date Issued
2022-06-01
Date Acceptance
2022-01-01
Citation
Journal of Magnetic Resonance Open, 2022, 10-11
URI
URL
DOI
ISSN
2666-4410
Publisher
Elsevier
Journal / Book Title
Journal of Magnetic Resonance Open
Volume
10-11
Copyright Statement
© 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
License URL
Identifier
10.1016/j.jmro.2022.100033
Subjects
Background-free
Biochemical Research Methods
Biochemistry & Molecular Biology
CP
dDNP
DNP
Hyperpolarization
Life Sciences & Biomedicine
NMR
Physical Sciences
Physics
Physics, Atomic, Molecular & Chemical
Science & Technology
Spectroscopy
Technology
Publication Status
Published
Article Number
100033
Date Publish Online
2022-01-10