In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass
Author(s)
Type
Journal Article
Abstract
Since lithium (Li+) plays roles in angiogenesis, the localized and controlled release of Li+ ions from bioactive glasses (BGs) represents a promising alternative therapy for the regeneration and repair of tissues with a high degree of vascularization. Here, microparticles from a base 45S5 BG composition containing (wt %) 45% SiO2, 24.5% Na2O, 24.5% CaO, and 6% P2O5, in which Na2O was partially substituted by 5% Li2O (45S5.5Li), were obtained. The results demonstrate that human umbilical vein endothelial cells (HUVECs) have greater migratory and proliferative response and ability to form tubules in vitro after stimulation with the ionic dissolution products (IDPs) of the 45S5.5Li BG. The results also show the activation of the canonical Wnt/β-catenin pathway and the increase in expression of proangiogenic cytokines insulin like growth factor 1 (IGF1) and transforming growth factor beta (TGFβ). We conclude that the IDPs of 45S5.5Li BG would act as useful inorganic agents to improve tissue repair and regeneration, ultimately stimulating HUVECs behavior in the absence of exogenous growth factors.
Date Issued
2017-07-03
Date Acceptance
2017-06-29
Citation
Materials, 2017, 10 (7)
ISSN
1996-1944
Publisher
MDPI AG
Journal / Book Title
Materials
Volume
10
Issue
7
Copyright Statement
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).
License URL
Subjects
Science & Technology
Technology
Materials Science, Multidisciplinary
Materials Science
endothelial cell
lithium
bioactive glass
WNT SIGNALING PATHWAY
MARROW STROMAL CELLS
OSTEOGENIC DIFFERENTIATION
BIPOLAR DISORDER
BETA-CATENIN
STEM-CELLS
STIMULATE ANGIOGENESIS
GROWTH-FACTORS
SOL-GEL
BONE
Publication Status
Published
Article Number
740