Coronavirus JHM: nucleotide sequence of the mRNA that encodes nucleocapsid protein
Author(s)
SKINNER, MA
SIDDELL, SG
Type
Journal Article
Abstract
A DNA copy of the mRNA that encodes the nucleocapsid protein of Mouse Hepatitis Virus JHM has been cloned into pAT153. The DNA copy specifically inhibited the synthesis in vitro of the nucleocapsid protein. The cDNA was subcloned into M13 vectors and the entire sequence, 1767 bases Including a 15 base terminal poly (A) tract, has been determined by chain-terminator sequencing. The sequence contained an open-reading frame that could encode a basic protein of mol.wt. 49700. From the predicted sequence it was apparent that the nucleocapsid protein has 5 basic regions, two of which are located near the middle of the sequence, a serine-r1ch region was also located, a feature which may be of functional importance as the nucleocapsid protein is phosphorylated at serine residues. The carboxy terminus of the nucleocapsid protein was found to be acidic. The 5' non-coding sequence contained a triple repeat of the pentamer AATCT, a structural feature which may play a significant role during the production of subgenomic viral mRNAs.
Date Issued
1983-08-11
Date Acceptance
1983-08-01
Citation
Nucleic Acids Research, 1983, 11 (15), pp.5045-5054
ISSN
0305-1048
Publisher
Oxford University Press
Start Page
5045
End Page
5054
Journal / Book Title
Nucleic Acids Research
Volume
11
Issue
15
Copyright Statement
© 1983 IRL Press Limited
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:A1983RC32300004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Publication Status
Published
Date Publish Online
1983-08-11