MicroRNA detection by DNA-mediated liposome fusion
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Membrane fusion is a process of fundamental importance in biological systems that involves highly selective recognition mechanisms for the trafficking of molecular and ionic cargos. Mimicking natural membrane fusion mechanisms for the purpose of biosensor development holds great potential for amplified detection because relatively few highly discriminating targets lead to fusion and an accompanied engagement of a large payload of signal-generating molecules. In this work, sequence-specific DNA-mediated liposome fusion is used for the highly selective detection of microRNA. The detection of miR-29a, a known flu biomarker, is demonstrated down to 18 nm within 30 min with high specificity by using a standard laboratory microplate reader. Furthermore, one order of magnitude improvement in the limit of detection is demonstrated by using a novel imaging technique combined with an intensity fluctuation analysis, which is coined two-color fluorescence correlation microscopy.
Date Issued
2018-01-15
Date Acceptance
2017-11-30
Citation
ChemBioChem, 2018, 19 (5), pp.434-438
ISSN
1439-4227
Publisher
Wiley
Start Page
434
End Page
438
Journal / Book Title
ChemBioChem
Volume
19
Issue
5
Copyright Statement
© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
Rosetrees Trust
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Grant Number
M216
EP/K031953/1
EP/K020641/1
Subjects
FRET
biosensors
liposomes
membrane fusion
nucleic acids
0304 Medicinal And Biomolecular Chemistry
0601 Biochemistry And Cell Biology
Organic Chemistry
Publication Status
Published