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Combining mGRASP and optogenetics enables high-resolution functional mapping of descending cortical projections
File(s)
Author(s)
Song, Jun Ho
Lucaci, Diana
Calangiu, Ioana
Brown, Matthew TC
Park, Jin Sung
Type
Journal Article
Abstract
We have applied optogenetics and mGRASP, a light microscopy technique that labels synaptic contacts, to map the number and strength of defined corticocollicular (CC) connections. Using mGRASP, we show that CC projections form small, medium, and large synapses, and both the number and the distribution of synapse size vary among the IC regions. Using optogenetics, we show that low-frequency stimulation of CC axons expressing channelrhodopsin produces prolonged elevations of the CC miniature EPSC (mEPSC) rate. Functional analysis of CC mEPSCs reveals small-, medium-, and large-amplitude events that mirror the synaptic distributions observed with mGRASP. Our results reveal that descending ipsilateral projections dominate CC feedback via an increased number of large synaptic contacts, especially onto the soma of IC neurons. This study highlights the feasibility of combining microscopy (i.e., mGRASP) and optogenetics to reveal synaptic weighting of defined projections at the level of single neurons, enabling functional connectomic mapping in diverse neural circuits.
Date Issued
2018-07-24
Date Acceptance
2018-06-18
Citation
Cell Reports, 2018, 24 (4), pp.1071-1080
URI
DOI
ISSN
2211-1247
Publisher
Elsevier
Start Page
1071
End Page
1080
Journal / Book Title
Cell Reports
Volume
24
Issue
4
Copyright Statement
© 2018 The Author(s). This is an open access article under
the CC-BY license (http://creativecommons.org/licenses/by/4.0/).
Sponsor
Medical Research Council (MRC)
Commission of the European Communities
Human Frontier Science Program
Medical Research Foundation
Biotechnology and Biological Sciences Research Council (BBSRC)
Grant Number
G1000512
PIEF-GA-2012-331858
RGY089/2012
C0488
BB/N008871/1
Subjects
corticofugal
functional connectomics
synapse
synaptic integration
Publication Status
Published
Date Publish Online
2018-07-24