OBJECTIVES: To compare efficiency, ease of use, and user satisfaction of two methods of transvaginal ultrasound probe high-level disinfection: Ultraviolet (UV-C) (Germitec Hypernova Chronos) and a chlorine dioxide multi-step wipe system (Tristel Trio). METHODS: This was a prospective survey study. UV-C units were introduced into a busy Early Pregnancy Assessment Unit and compared with a multi-wipe system. Healthcare professionals (HCP's) measured time taken to complete a cycle of disinfection before each patient with the system allocated to that room using a stopwatch and recorded their responses using a quick response (QR) code-linked survey. Additional necessary tasks that could be completed before seeing the next patient as probe disinfection was ongoing were also documented. Using another QR code-linked survey, data on ease of use, satisfaction of the system used, and preference was collected. A section for free-text comments was then completed. RESULTS: Disinfection using UV-C (n=331) was 60% faster than the chlorine dioxide multi-wipe system (n=332) (101 vs 250 seconds, p<0.0001). A greater number of tasks during probe disinfection were completed when using UV-C, saving a further 74 seconds per patient (p<0.0001). HCP's using UV-C (n=71) reported greater ease of use (10 vs 3/10, p<0.0001) and satisfaction (10 vs 2/10, p<0.0001) than those using the multi-wipe system (n=43). HCP's reported that the chlorine dioxide system was time-consuming and environmentally unfriendly, whilst the UV-C system was efficient and easy to use. 98% HCP's preferred using the UV-C system. CONCLUSIONS: UV-C technology was more efficient and allowed more essential tasks to be completed during disinfection. For a four-hour ultrasound list of 15 patients, we calculated this would amount to 55 minutes 45 seconds extra time available. HCP's found UV-C preferable and easier to use. This article is protected by copyright. All rights reserved.