Biodegradable silicon nanoneedles delivering nucleic acids intracellularly induce localized in vivo neovascularization.
File(s)
Accepted version
Supporting information
Author(s)
Type
Journal Article
Abstract
The controlled delivery of nucleic acids to selected tissues remains an inefficient process mired by low transfection efficacy, poor scalability because of varying efficiency with cell type and location, and questionable safety as a result of toxicity issues arising from the typical materials and procedures employed. High efficiency and minimal toxicity in vitro has been shown for intracellular delivery of nuclei acids by using nanoneedles, yet extending these characteristics to in vivo delivery has been difficult, as current interfacing strategies rely on complex equipment or active cell internalization through prolonged interfacing. Here, we show that a tunable array of biodegradable nanoneedles fabricated by metal-assisted chemical etching of silicon can access the cytosol to co-deliver DNA and siRNA with an efficiency greater than 90%, and that in vivo the nanoneedles transfect the VEGF-165 gene, inducing sustained neovascularization and a localized sixfold increase in blood perfusion in a target region of the muscle.
Date Issued
2015-05-01
Date Acceptance
2015-02-11
Citation
Nature Materials, 2015, 14 (5), pp.532-539
ISSN
1476-1122
Publisher
Nature Research
Start Page
532
End Page
539
Journal / Book Title
Nature Materials
Volume
14
Issue
5
Copyright Statement
© 2015, Rights Managed by Nature Publishing Group
Sponsor
Commission of the European Communities
Wellcome Trust
Identifier
PII: nmat4249
Grant Number
ERC-2013-CoG-616417
098411/Z/12/Z
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Physical
Materials Science, Multidisciplinary
Physics, Applied
Physics, Condensed Matter
Chemistry
Materials Science
Physics
POROUS SILICON
GENE-TRANSFER
GROWTH-FACTORS
LIVING CELLS
DRUG-DELIVERY
ELECTROPORATION
PLATFORM
MICROVESICLES
RNA
THERAPEUTICS
Animals
Humans
Mice
Muscle, Skeletal
Nanostructures
Needles
Neovascularization, Physiologic
Plasmids
Silicon
Transfection
Vascular Endothelial Growth Factor A
Muscle, Skeletal
Animals
Humans
Mice
Silicon
Vascular Endothelial Growth Factor A
Transfection
Needles
Neovascularization, Physiologic
Plasmids
Nanostructures
Nanoscience & Nanotechnology
Publication Status
Published
Date Publish Online
2015-03-30