Inline mechano-vibration holography for simultaneous phase and elasticity mapping of soft samples
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Off-axis holography enables single-shot phase retrieval but reduces spatial bandwidth, while in-line phase-shifting interferometry preserves bandwidth yet requires reference-path stepping and is sensitive to drift, limiting dynamic measurements. Moreover, viscoelastic mapping is rarely available from the same holographic measurement. We propose vibration-encoded in-line Mach-Zehnder holography for simultaneous thickness and viscoelasticity mapping of soft samples. Twelve holograms acquired over one vibration cycle are analyzed using Bessel-based harmonic inversion and robust regression to recover the static phase, modulation depth, and phase lag, yielding thickness and Kelvin-Voigt storage and loss modulus maps (E′, E″). Simulations recover E′ and E′′ to within ∼2% across a wide E′′/E′ range and achieve sub-micron thickness error over 20-45 μm beads. Experiments on calibrated polyacrylamide beads show sub-micron thickness repeatability (median ∼0.57 μm over 40 repeats) and stiffness estimates typically within 10% of ground truth, and we further demonstrate the approach on adherent MCF-7 cells.
Date Issued
2026-02-01
Date Acceptance
2026-01-14
Citation
Biomedical Optics Express, 2026, 17 (2), pp.901-915
ISSN
2156-7085
Publisher
Optica Publishing Group
Start Page
901
End Page
915
Journal / Book Title
Biomedical Optics Express
Volume
17
Issue
2
Copyright Statement
© 2026 Published by Optica Publishing Group under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/41693886
PII: boe-17-2-901
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2026-01-23