Detection of microRNA biomarkers via inhibition of DNA-mediated liposome fusion
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Published version
Author(s)
Type
Journal Article
Abstract
We report the specific and sensitive detection of microRNA using an inverse DNA-mediated liposome fusion assay. This assay is homogeneous, and does not require washing, separation, or enzyme-associated amplification steps. By fine-tuning the surface functionalisation of the liposomes, liposome concentration, and assay temperature, we demonstrated a sub-nanomolar limit of detection for the target.
Date Issued
2019-02-01
Date Acceptance
2018-11-14
Citation
Nanoscale Advances, 2019, 1 (2), pp.532-536
ISSN
2516-0230
Publisher
Royal Society of Chemistry
Start Page
532
End Page
536
Journal / Book Title
Nanoscale Advances
Volume
1
Issue
2
Copyright Statement
© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 3.0 Unported Licence ( https://creativecommons.org/licenses/by/3.0/ )
Sponsor
Engineering & Physical Science Research Council (E
Engineering & Physical Science Research Council (EPSRC)
Grant Number
EP/K031953/1
EP/K020641/1
Subjects
Science & Technology
Physical Sciences
Technology
Chemistry, Multidisciplinary
Nanoscience & Nanotechnology
Materials Science, Multidisciplinary
Chemistry
Science & Technology - Other Topics
Materials Science
ARRAYS
Publication Status
Published
Date Publish Online
2018-11-21