"Pathotyping" Multiplex PCR Assay for haemophilus parasuis: a tool for prediction of virulence
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Haemophilus parasuis is a diverse bacterial species that is found in the upper respiratory tracts of pigs and can also cause Glässer's disease and pneumonia. A previous pangenome study of H. parasuis identified 48 genes that were associated with clinical disease. Here, we describe the development of a generalized linear model (termed a pathotyping model) to predict the potential virulence of isolates of H. parasuis based on a subset of 10 genes from the pangenome. A multiplex PCR (mPCR) was constructed based on these genes, the results of which were entered into the pathotyping model to yield a prediction of virulence. This new diagnostic mPCR was tested on 143 field isolates of H. parasuis that had previously been whole-genome sequenced and a further 84 isolates from the United Kingdom from cases of H. parasuis-related disease in pigs collected between 2013 and 2014. The combination of the mPCR and the pathotyping model predicted the virulence of an isolate with 78% accuracy for the original isolate collection and 90% for the additional isolate collection, providing an overall accuracy of 83% (81% sensitivity and 93% specificity) compared with that of the “current standard” of detailed clinical metadata. This new pathotyping assay has the potential to aid surveillance and disease control in addition to serotyping data.
Date Issued
2017-06-14
Date Acceptance
2017-05-28
Citation
Journal of Clinical Microbiology, 2017, 55 (9), pp.2617-2628
ISSN
0095-1137
Publisher
American Society for Microbiology
Start Page
2617
End Page
2628
Journal / Book Title
Journal of Clinical Microbiology
Volume
55
Issue
9
Copyright Statement
© 2017 American Society for Microbiology.
Sponsor
Biotechnology and Biological Sciences Research Council (BBSRC)
Pfizer Limited (UK)
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000408223700009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Grant Number
BB/G018553/1
N/A
Subjects
Science & Technology
Life Sciences & Biomedicine
Microbiology
Haemophilus parasuis
virulence factors
pathotyping
molecular diagnostics
GLASSERS-DISEASE
ACTINOBACILLUS-PLEUROPNEUMONIAE
INDIRECT HEMAGGLUTINATION
PIGS
STRAINS
GENE
DIVERSITY
INFECTION
SEROVAR
SWINE
Publication Status
Published