A modular, dynamic, DNA-based platform for regulating cargo distribution and transport between lipid domains
File(s)
Published version
Author(s)
Rubio, Roger
Eizagirre Barker, Simone
Walczak, Michal
Cicuta, Pietro
Di Michele, Lorenzo
Type
Journal Article
Abstract
Cell membranes regulate the distribution of biological machinery between phase-separated lipid domains to facilitate key processes including signaling and transport, which are among the life-like functionalities that bottom-up synthetic biology aims to replicate in artificial-cellular systems. Here, we introduce a modular approach to program partitioning of amphiphilic DNA nanostructures in coexisting lipid domains. Exploiting the tendency of different hydrophobic “anchors” to enrich different phases, we modulate the lateral distribution of our devices by rationally combining hydrophobes and by changing nanostructure size and topology. We demonstrate the functionality of our strategy with a bioinspired DNA architecture, which dynamically undergoes ligand-induced reconfiguration to mediate cargo transport between domains via lateral redistribution. Our findings pave the way to next-generation biomimetic platforms for sensing, transduction, and communication in synthetic cellular systems.
Date Issued
2021-04-14
Date Acceptance
2021-02-26
Citation
Nano Letters, 2021, 21 (7), pp.2800-2808
ISSN
1530-6984
Publisher
American Chemical Society
Start Page
2800
End Page
2808
Journal / Book Title
Nano Letters
Volume
21
Issue
7
Copyright Statement
© 2021 The Authors. Published by American Chemical Society. This article is open access under a Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/)
License URL
Sponsor
Commission of the European Communities
The Royal Society
Grant Number
851667
UF160152
Subjects
DNA nanotechnology
artificial cells
biomimicry
lipid domains
lipid phase separation
partitioning
synthetic membranes
Nanoscience & Nanotechnology
Publication Status
Published
Date Publish Online
2021-03-18