CD71 targeting boosts immunogenicity of sublingually delivered influenza haemagglutinin antigen and protects against viral challenge in mice.
File(s)
Accepted version
Author(s)
Mann, JF
Tregoning, JS
Aldon, Y
Shattock, RJ
McKay, PF
Type
Journal Article
Abstract
The delivery of vaccines to the sublingual mucosa is an attractive prospect due to the ease and acceptability of such an approach. However, novel adjuvant and delivery approaches are required to optimally vaccinate at this site. We have previously shown that conjugation of protein antigen to the iron transport molecule, transferrin, can significantly enhance mucosal immune responses. We tested whether conjugating influenza haemagglutinin to transferrin could improve the immune response to sublingually delivered antigen. Transferrin conjugated haemagglutinin induced a significant antibody and T cell response in both naïve animals and previously immunized animals. The immune response generated was able to protect mice against influenza virus challenge. Sublingually administered antigen dispersed more widely through the gastro-intestinal tract than intranasally delivered antigen and transferrin conjugation had a more marked effect on sublingually delivered antigen than intranasal immunisation. From these studies we conclude that transferrin conjugation of antigen is effective at boosting immune responses to sublingually delivered antigen and may be an attractive approach for influenza vaccines, particularly when mass campaigns are required.
Date Issued
2016-04-16
Date Acceptance
2016-04-07
Citation
Journal of Controlled Release, 2016, 232, pp.75-82
ISSN
1873-4995
Publisher
Elsevier
Start Page
75
End Page
82
Journal / Book Title
Journal of Controlled Release
Volume
232
Copyright Statement
© 2016 Published by Elsevier B.V. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Identifier
PII: S0168-3659(16)30202-4
Subjects
Immune response
Influenza
Oral
Sublingual
Targeting
Transferrin
Pharmacology & Pharmacy
0903 Biomedical Engineering
1115 Pharmacology And Pharmaceutical Sciences
Publication Status
Published