The Insulin Receptor Is Colocalized With the TRPV1 Nociceptive Ion Channel and Neuropeptides in Pancreatic Spinal and Vagal Primary Sensory Neurons
File(s)
Published version
Author(s)
Nagy, I
Bence, Lazar
Gabor, Jancso
Orsolya, Oszlacs
Peter, Santha
Type
Journal Article
Abstract
Objectives Recent observations demonstrated the expression of the insulin receptor (InsR) and its functional interaction with the transient receptor potential vanilloid type 1 receptor (TRPV1) in sensory ganglion neurons. Because sensory nerves are implicated in pancreatic inflammatory processes, we studied the colocalization of the InsR with TRPV1 and proinflammatory neuropeptides in spinal and vagal pancreatic afferent neurons.
Methods Immunohistochemistry and quantitative morphometry were used to analyze the expression of TRPV1, InsR, substance P (SP), and calcitonin gene-related peptide (CGRP) in retrogradely labeled pancreatic dorsal root ganglion (DRG) and nodose ganglion (NG) neurons.
Results The proportions of retrogradely labeled pancreatic TRPV1-, InsR-, SP-, and CGRP-immunoreactive neurons amounted to 68%, 48%, 33%, and 54% in DRGs and 64%, 49%, 40%, and 25% in the NGs. Of the labeled DRG and NG neurons, 23% and 35% showed both TRPV1 and InsR immunoreactivity. Colocalization of the InsR with SP or CGRP was demonstrated in 14% and 28% of pancreatic DRG and 24% and 8% of pancreatic NG neurons.
Conclusions The present findings provide morphological basis for possible functional interactions among the nociceptive ion channel TRPV1, the InsR, and the proinflammatory neuropeptides SP and CGRP expressed by pancreatic DRG and NG neurons.
Methods Immunohistochemistry and quantitative morphometry were used to analyze the expression of TRPV1, InsR, substance P (SP), and calcitonin gene-related peptide (CGRP) in retrogradely labeled pancreatic dorsal root ganglion (DRG) and nodose ganglion (NG) neurons.
Results The proportions of retrogradely labeled pancreatic TRPV1-, InsR-, SP-, and CGRP-immunoreactive neurons amounted to 68%, 48%, 33%, and 54% in DRGs and 64%, 49%, 40%, and 25% in the NGs. Of the labeled DRG and NG neurons, 23% and 35% showed both TRPV1 and InsR immunoreactivity. Colocalization of the InsR with SP or CGRP was demonstrated in 14% and 28% of pancreatic DRG and 24% and 8% of pancreatic NG neurons.
Conclusions The present findings provide morphological basis for possible functional interactions among the nociceptive ion channel TRPV1, the InsR, and the proinflammatory neuropeptides SP and CGRP expressed by pancreatic DRG and NG neurons.
Date Issued
2018-01-01
Date Acceptance
2017-10-18
Citation
Pancreas, 2018, 47 (1), pp.110-115
ISSN
0885-3177
Publisher
Lippincott, Williams & Wilkins
Start Page
110
End Page
115
Journal / Book Title
Pancreas
Volume
47
Issue
1
Subjects
Science & Technology
Life Sciences & Biomedicine
Gastroenterology & Hepatology
insulin receptor
neuropeptides
pancreas
primary afferent neurons
retrograde labeling
TRPV1
GENE-RELATED PEPTIDE
PRIMARY AFFERENT NEURONS
ROOT GANGLION NEURONS
SUBSTANCE-P
CAPSAICIN RECEPTOR
RAT PANCREAS
PAIN PATHWAY
INFLAMMATION
INNERVATION
TISSUE
1103 Clinical Sciences
Publication Status
Published