Large-scale production of membrane proteins in Pichia pastoris: the production of G protein-coupled receptors as a case study.
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
One of the major advantages of using Pichia pastoris is that it is readily adapted to large-scale culture in bioreactors. Bioreactors allow precise regulation of cell growth parameters increasing both yields and reproducibility of the culture. P. pastoris cultures grow to very high cell densities which helps minimise culture volume and facilitates downstream processing of the sample. Here, we provide protocols for the large-scale production of the human adenosine A(2A) receptor (A(2A)R) and provide some details of how bioreactor cultures can be used for optimisation of expression of the human dopamine D2 receptor (D2DR).
Date Issued
2012
Citation
Methods Mol Biol, 2012, 866, pp.197-207
ISSN
1064-3745
Publisher
Springer
Start Page
197
End Page
207
Journal / Book Title
Methods Mol Biol
Volume
866
Copyright Statement
© Springer Science+Business Media, LLC 2012. The oringinal publication is available at www.springerlink.com
Description
03.12.12 KB. Accepted version ok to add to Spiral. Springer policy
Subjects
Bioreactors
Humans
Pichia
Receptors, Adenosine A2
Receptors, G-Protein-Coupled
Coverage Spatial
United States