Adaptive multiphoton endomicroscopy through a dynamically deformed multicore optical fiber using proximal detection
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Accepted version
Published version
Author(s)
Type
Journal Article
Abstract
This paper demonstrates multiphoton excited fluorescence
imaging through a polarisation maintaining multicore fiber (PM-MCF)
while the fiber is dynamically deformed using all-proximal detection.
Single-shot proximal measurement of the relative optical path lengths of all
the cores of the PM-MCF in double pass is achieved using a Mach-Zehnder
interferometer read out by a scientific CMOS camera operating at 416 Hz.
A non-linear least squares fitting procedure is then employed to determine
the deformation-induced lateral shift of the excitation spot at the distal tip of
the PM-MCF. An experimental validation of this approach is presented that
compares the proximally measured deformation-induced lateral shift in
focal spot position to an independent distally measured ground truth. The
proximal measurement of deformation-induced shift in focal spot position is
applied to correct for deformation-induced shifts in focal spot position
during raster-scanning multiphoton excited fluorescence imaging.
imaging through a polarisation maintaining multicore fiber (PM-MCF)
while the fiber is dynamically deformed using all-proximal detection.
Single-shot proximal measurement of the relative optical path lengths of all
the cores of the PM-MCF in double pass is achieved using a Mach-Zehnder
interferometer read out by a scientific CMOS camera operating at 416 Hz.
A non-linear least squares fitting procedure is then employed to determine
the deformation-induced lateral shift of the excitation spot at the distal tip of
the PM-MCF. An experimental validation of this approach is presented that
compares the proximally measured deformation-induced lateral shift in
focal spot position to an independent distally measured ground truth. The
proximal measurement of deformation-induced shift in focal spot position is
applied to correct for deformation-induced shifts in focal spot position
during raster-scanning multiphoton excited fluorescence imaging.
Date Issued
2016-09-19
Date Acceptance
2016-07-15
Citation
Optics Express, 2016, 24 (19), pp.21474-21484
ISSN
1094-4087
Publisher
Optical Society of America
Start Page
21474
End Page
21484
Journal / Book Title
Optics Express
Volume
24
Issue
19
Copyright Statement
Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.
Further distribution of this work must maintain attribution to the author(s) and the published article’s title,
journal citation, and DOI.
Further distribution of this work must maintain attribution to the author(s) and the published article’s title,
journal citation, and DOI.
License URL
Sponsor
Engineering & Physical Science Research Council (EPSRC)
Grant Number
EP/K020102/1
Subjects
Optics
0205 Optical Physics
1005 Communications Technologies
0906 Electrical And Electronic Engineering
Publication Status
Published