Criteria for chamber-specific categorisation of human cardiac myocytes derived from pluripotent stem cells
File(s)
Published version
Author(s)
Kane, C
Terracciano, CMN
Type
Journal Article
Abstract
Human pluripotent stem cell-derived cardiomyocytes (PSC-CMs) have great potential application in almost all areas of cardiovascular research. A current major goal of the field is to build on the past success of differentiation strategies to produce CMs with the properties of those originating from the different chambers of the adult human heart. With no anatomical origin or developmental pathway to draw on, the question of how to judge the success of such approaches and assess the chamber specificity of PSC-CMs has become increasingly important; commonly used methods have substantial limitations and are based on limited evidence to form such an assessment. In this article, we discuss the need for chamber-specific PSC-CMs in a number of areas as well as current approaches used to assess these cells on their likeness to those from different chambers of the heart. Furthermore, describing in detail the structural and functional features that distinguish the different chamber-specific human adult cardiac myocytes, we propose an evidence-based tool to aid investigators in the phenotypic characterization of differentiated PSC-CMs. Stem Cells 2017
Date Issued
2017-06-27
Date Acceptance
2017-05-12
Citation
Stem Cells, 2017, 35 (8), pp.1881-1897
ISSN
1549-4918
Publisher
Wiley
Start Page
1881
End Page
1897
Journal / Book Title
Stem Cells
Volume
35
Issue
8
Copyright Statement
© 2017 The Authors Stem Cells published by Wiley Periodicals, Inc. on behalf of AlphaMed Press
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
British Heart Foundation
British Heart Foundation
Grant Number
RM/13/1/30157
FS/13/46/30282
Subjects
Cardiac toxicology models
Chamber specificity
Heart
Heart disease models
Induced pluripotent stem cell-derived cardiac myocytes
Induced pluripotent stem cells
Maturation
06 Biological Sciences
10 Technology
11 Medical And Health Sciences
Immunology
Publication Status
Published