High-speed detection of DNA Translocation in Nanopipettes
File(s)
Accepted version
Published version
Author(s)
Type
Journal Article
Abstract
We present a high-speed electrical detection scheme based on a custom-designed CMOS amplifier which allows the analysis of DNA translocation in glass nanopipettes on a microsecond timescale. Translocation of different DNA lengths in KCl electrolyte provides a scaling factor of the DNA translocation time equal to p = 1.22, which is different from values observed previously with nanopipettes in LiCl electrolyte or with nanopores. Based on a theoretical model involving electrophoresis, hydrodynamics and surface friction, we show that the experimentally observed range of p-values may be the result of, or at least be affected by DNA adsorption and friction between the DNA and the substrate surface.
Date Issued
2016-03-11
Date Acceptance
2016-03-10
Citation
Nanoscale, 2016, 8, pp.7604-7611
ISSN
2040-3372
Publisher
Royal Society of Chemistry
Start Page
7604
End Page
7611
Journal / Book Title
Nanoscale
Volume
8
Copyright Statement
Raquel L. Fraccari,a Pietro Ciccarella,b Azadeh Bahrami,a Marco Carminati,b Giorgio Ferrarib and Tim Albrecht*a
Show Affiliations
Nanoscale, 2016,8, 7604-7611
DOI: 10.1039/C5NR08634E
Received 04 Dec 2015, Accepted 10 Mar 2016
First published online 11 Mar 2016 This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Show Affiliations
Nanoscale, 2016,8, 7604-7611
DOI: 10.1039/C5NR08634E
Received 04 Dec 2015, Accepted 10 Mar 2016
First published online 11 Mar 2016 This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Subjects
Nanoscience & Nanotechnology
10 Technology
02 Physical Sciences
03 Chemical Sciences
Publication Status
Published