Continuous measurement of lactate concentration in human subjects through direct electron transfer from enzymes to microneedle electrodes
Author(s)
Type
Journal Article
Abstract
Microneedle lactate sensors may be used to continuously measure lactate concentration in the interstitial fluid in a minimally invasive and pain-free manner. First- and second-generation enzymatic sensors produce a redox-active product that is electrochemically sensed at the electrode surface. Direct electron transfer enzymes produce electrons directly as the product of enzymatic action; in this study, a direct electron transfer enzyme specific to lactate has been immobilized onto a microneedle surface to create lactate-sensing devices that function at low applied voltages (0.2 V). These devices have been validated in a small study of human volunteers; lactate concentrations were raised and lowered through physical exercise and subsequent rest. Lactazyme microneedle devices show good agreement with concurrently obtained and analyzed serum lactate levels.
Date Issued
2023-04-28
Date Acceptance
2023-03-14
Citation
ACS Sensors, 2023, 8 (4), pp.1639-1647
ISSN
2379-3694
Publisher
American Chemical Society
Start Page
1639
End Page
1647
Journal / Book Title
ACS Sensors
Volume
8
Issue
4
Copyright Statement
Copyright © 2023 The Authors. Published by American Chemical Society. This publication is licensed under
CC-BY 4.0.
CC-BY 4.0.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000959722300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
biosensor
BIOSENSORS
Chemistry
Chemistry, Analytical
Chemistry, Multidisciplinary
continuous monitoring
direct electron transfer enzyme
GLUCOSE
in-human study
lactate
microneedles
Nanoscience & Nanotechnology
Physical Sciences
Science & Technology
Science & Technology - Other Topics
SEPSIS
SEPTIC SHOCK
Publication Status
Published
Date Publish Online
2023-03-26