Hyphenated MS–Based Targeted approaches in metabolomics
File(s)
Accepted version
Author(s)
Wilson, ID
Begou, O
Gika, HG
Theodoridis, G
Type
Journal Article
Abstract
While global metabolic profiling (untargeted metabolomics) has been the center of much interest and research activity in the past few decades, more recently targeted metabolomics approaches have begun to gain ground. These analyses are, to an extent, more hypothesis-driven, as they focus on a set of pre-defined metabolites and aim towards their determination, often to the point of absolute quantification. The continuous development of the technological platforms used in these studies facilitates the analysis of large numbers of well-characterized metabolites present in complex matrices. The present review describes recent developments in the hyphenated chromatographic methods most often applied in targeted metabolomic/lipidomic studies (LC-MS/MS, CE-MS/MS, and GC-MS/MS), highlighting applications in the life and food/plant sciences. The review also underlines practical challenges–limitations that appear in such approaches.
Date Issued
2017-07-19
Date Acceptance
2017-07-19
Citation
Analyst, 2017, 142, pp.3079-3100
ISSN
1364-5528
Publisher
Royal Society of Chemistry
Start Page
3079
End Page
3100
Journal / Book Title
Analyst
Volume
142
Copyright Statement
© The Royal Society of Chemistry 2017
Subjects
0301 Analytical Chemistry
0399 Other Chemical Sciences
Analytical Chemistry
Publication Status
Published
Date Publish Online
2017-07-19