Xenon protects against blast-induced traumatic brain injury in an in vitro model
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
The aim of this study was to evaluate the neuroprotective efficacy of the inert gas xenon as a treatment for patients with blast-induced traumatic brain injury in an in vitro laboratory model. We developed a novel blast traumatic brain injury model using C57BL/6N mouse organotypic hippocampal brain-slice cultures exposed to a single shockwave, with the resulting injury quantified using propidium iodide fluorescence. A shock tube blast generator was used to simulate open field explosive blast shockwaves, modeled by the Friedlander waveform. Exposure to blast shockwave resulted in significant (p < 0.01) injury that increased with peak-overpressure and impulse of the shockwave, and which exhibited a secondary injury development up to 72 h after trauma. Blast-induced propidium iodide fluorescence overlapped with cleaved caspase-3 immunofluorescence, indicating that shock-wave–induced cell death involves apoptosis. Xenon (50% atm) applied 1 h after blast exposure reduced injury 24 h (p < 0.01), 48 h (p < 0.05), and 72 h (p < 0.001) later, compared with untreated control injury. Xenon-treated injured slices were not significantly different from uninjured sham slices at 24 h and 72 h. We demonstrate for the first time that xenon treatment after blast traumatic brain injury reduces initial injury and prevents subsequent injury development in vitro. Our findings support the idea that xenon may be a potential first-line treatment for those with blast-induced traumatic brain injury.
Date Issued
2018-04-15
Date Acceptance
2017-12-28
Citation
Journal of Neurotrauma, 2018, 35 (8), pp.1037-1044
ISSN
0897-7151
Publisher
Mary Ann Liebert
Start Page
1037
End Page
1044
Journal / Book Title
Journal of Neurotrauma
Volume
35
Issue
8
Copyright Statement
©
Rita Campos-Pires et al., 2018; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative
Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium,
provided
the original work is properly credited.
Rita Campos-Pires et al., 2018; Published by Mary Ann Liebert, Inc. This Open Access article is distributed under the terms of the Creative
Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium,
provided
the original work is properly credited.
Sponsor
Air Products And Chemicals Inc
Air Products And Chemicals Inc
Carburos Metalicos S.E.C.M.S.A
Carburos Metalicos S.E.C.M.S.A
Medical Research Council (MRC)
Westminster Medical School Research Trust
Royal Centre for Defence Medicine
The Royal British Legion
Medical Research Council (MRC)
Medical Research Council (MRC)
Grant Number
084-344-P
084-344-P
n/a
N/A
G0901892
JRC 1-/11 PHD 001
20120229-DMSRASG/Xenon
Centre for Blast Injury Studie
MC_PC_13064
MR/N027736/1
Subjects
TBI
blast traumatic brain injury
blast-induced neurotrauma
neuroprotection
primary blast injury
xenon
1103 Clinical Sciences
1109 Neurosciences
Neurology & Neurosurgery
Publication Status
Published