Luminescent surfaces with tailored angular emission for compact dark-field imaging devices
File(s)
Accepted version
Author(s)
Type
Journal Article
Abstract
Dark-field microscopy is a standard imaging technique widely employed in biology that provides high image contrast for a broad range of unstained specimens1. Unlike bright-field microscopy, it accentuates high spatial frequencies and can therefore be used to emphasize and resolve small features. However, the use of dark-field microscopy for reliable analysis of blood cells, bacteria, algae and other marine organisms often requires specialized, bulky microscope systems, as well as expensive additional components, such as dark-field-compatible objectives or condensers2,3. Here, we propose to simplify and downsize dark-field microscopy equipment by generating the high-angle illumination cone required for dark-field microscopy directly within the sample substrate. We introduce a luminescent photonic substrate with a controlled angular emission profile and demonstrate its ability to generate high-contrast dark-field images of micrometre-sized living organisms using standard optical microscopy equipment. This new type of substrate forms the basis for miniaturized lab-on-chip dark-field imaging devices that are compatible with simple and compact light microscopes.
Date Issued
2020-05-01
Date Acceptance
2020-01-21
Citation
Nature Photonics, 2020, 14 (5), pp.310-315
ISSN
1749-4885
Publisher
Nature Research
Start Page
310
End Page
315
Journal / Book Title
Nature Photonics
Volume
14
Issue
5
Copyright Statement
© 2020 Springer Nature Limited.
Identifier
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000515476500002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Physical Sciences
Optics
Physics, Applied
Physics
MICROSCOPY
ILLUMINATION
PRECISION
SUBSTEPS
Publication Status
Published
Date Publish Online
2020-02-24