We report an improved measurement of the cosmic microwave background B-mode polarization power spectrum with the Polarbear experiment at 150 GHz. By adding new data collected during the second season of observations (2013–2014) to re-analyzed data from the first season (2012–2013), we have reduced twofold the band-power uncertainties. The band powers are reported over angular multipoles $500\leqslant {\ell }\leqslant 2100$, where the dominant B-mode signal is expected to be due to the gravitational lensing of E-modes. We reject the null hypothesis of no B-mode polarization at a confidence of 3.1σ including both statistical and systematic uncertainties. We test the consistency of the measured B-modes with the Λ Cold Dark Matter (ΛCDM) framework by fitting for a single lensing amplitude parameter A L relative to the Planck 2015 best-fit model prediction. We obtain ${A}_{L}={0.60}_{-0.24}^{+0.26}(\mathrm{stat}{)}_{-0.04}^{+0.00}(\mathrm{inst})$ ± 0.14(foreground) ± 0.04(multi), where ${A}_{L}=1$ is the fiducial ΛCDM value.