Molecular rotors: fluorescent sensors for microviscosity and conformation of biomolecules
Author(s)
Paez-Perez, Miguel
Kuimova, Marina K
Type
Journal Article
Abstract
The viscosity and crowding of biological environment are considered vital for the correct cellular function, and alterations in these parameters are known to underly a number of pathologies including diabetes, malaria, cancer and neurodegenerative diseases, to name a few. Over the last decades, fluorescent molecular probes termed molecular rotors proved extremely useful for exploring viscosity, crowding, and underlying molecular interactions in biologically relevant settings. In this review, we will discuss the basic principles underpinning the functionality of these probes and will review advances in their use as sensors for lipid order, protein crowding and conformation, temperature and non-canonical nucleic acid structures in live cells and other relevant biological settings.
Date Issued
2024-02-05
Date Acceptance
2023-10-01
Citation
Angewandte Chemie International Edition, 2024, 63 (6)
ISSN
1433-7851
Publisher
Wiley
Journal / Book Title
Angewandte Chemie International Edition
Volume
63
Issue
6
Copyright Statement
© 2023 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Identifier
https://onlinelibrary.wiley.com/doi/full/10.1002/anie.202311233
Subjects
Biophysics
Chemistry
Chemistry, Multidisciplinary
FLIM
G-QUADRUPLEXES
Lipid Membranes
LIPID-MEMBRANES
LIVE CELLS
LYSOSOMAL VISCOSITY
MICRO-ENVIRONMENTS
Microviscosity
Molecular Rotors
Physical Sciences
PLASMA-MEMBRANE VISCOSITY
PROBES DESIGN
PROTEIN AGGREGATION
Science & Technology
SOLVENT VISCOSITY
THIOFLAVIN-T
Publication Status
Published
Article Number
e202311233
Date Publish Online
2023-10-19