Vulpeculin: a novel and abundant lipocalin in the urine of the common brushtail possum, Trichosurus vulpecula
Author(s)
Type
Journal Article
Abstract
Lipocalins are a family of secreted proteins. They are capable of binding small lipophilic compounds and have been extensively studied for their role in chemosignalling in rodent urine. Urine of the common brushtail possum (Trichosurus vulpecula) contains a prominent glycoprotein of 20 kDa, expressed in both sexes. We have isolated this protein and determined its primary sequence by mass spectrometry, including the use of metabolic labelling to resolve the leucine/isoleucine isobaric ambiguity. The protein sequence was identified as a lipocalin, and phylogenetic analysis grouped the protein with other marsupial lipocalin sequences in a phylogenetic clade distinct from established cross-species lipocalin sub-families. The pattern of expression in possum urine and the similarity in sequence and structure to other lipocalins suggests this protein may have a role in brushtail possum chemosignalling.
Date Issued
2020-10
Date Acceptance
2020-09-10
Citation
Open Biology, 2020, 10 (10)
ISSN
2046-2441
Publisher
The Royal Society
Journal / Book Title
Open Biology
Volume
10
Issue
10
Copyright Statement
© 2020 The Authors.
Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
License URL
Identifier
https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000578418900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
Subjects
Biochemistry & Molecular Biology
brushtail possum
GLAND
LATE LACTATION
Life Sciences & Biomedicine
major urinary protein
mass spectrometry
metabolic labelling
MULTIPLE SEQUENCE ALIGNMENT
NEW-ZEALAND
ORONGORONGO VALLEY
PHYLOGENETIC ANALYSIS
POSSUM
PROTEIN
Science & Technology
Trichosurus vulpecula
TRICHOSURUS-VULPECULA
VOMERONASAL ORGAN
Publication Status
Published
Article Number
200218
Date Publish Online
2020-10-07