Raman spectroscopy reveals new insights into the zonal organization of native and tissue-engineered articular cartilage
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Tissue architecture is intimately linked with its functions, and loss of tissue organization is often associated with pathologies. The intricate depth-dependent extracellular matrix (ECM) arrangement in articular cartilage is critical to its biomechanical functions. In this study, we developed a Raman spectroscopic imaging approach to gain new insight into the depth-dependent arrangement of native and tissue-engineered articular cartilage using bovine tissues and cells. Our results revealed previously unreported tissue complexity into at least six zones above the tidemark based on a principal component analysis and k-means clustering analysis of the distribution and orientation of the main ECM components. Correlation of nanoindentation and Raman spectroscopic data suggested that the biomechanics across the tissue depth are influenced by ECM microstructure rather than composition. Further, Raman spectroscopy together with multivariate analysis revealed changes in the collagen, glycosaminoglycan and water distributions in tissue-engineered constructs over time. These changes were assessed using simple metrics that promise to instruct efforts towards the regeneration of a broad range of tissues with native zonal complexity and functional performance.
Date Issued
2016-11-16
Date Acceptance
2016-10-27
Citation
ACS Central Science, 2016, 2 (12), pp.885-895
ISSN
2374-7951
Publisher
American Chemical Society
Start Page
885
End Page
895
Journal / Book Title
ACS Central Science
Volume
2
Issue
12
Copyright Statement
© 2016 American Chemical Society. ACS AuthorChoice - This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
Sponsor
Wellcome Trust
Medical Research Council (MRC)
Commission of the European Communities
Medical Research Council (MRC)
Grant Number
088844/Z/09/Z
MR/K026666/1
ERC-2013-CoG-616417
RA26F7
Subjects
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
IN-VITRO
COLLAGEN
SCATTERING
MICROSCOPY
MICROSPECTROSCOPY
PROTEOGLYCAN
LIMITATIONS
SCAFFOLDS
BEHAVIOR
PROGRESS
Publication Status
Published