Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Despite having many key roles in cellular biology, directly imaging biologically important RNAs has been hindered by a lack of fluorescent tools equivalent to the fluorescent proteins available to study cellular proteins. Ideal RNA labelling systems must preserve biological function, have photophysical properties similar to existing fluorescent proteins, and be compatible with established live and fixed cell protein labelling strategies. Here, we report a microfluidics-based selection of three new high-affinity RNA Mango fluorogenic aptamers. Two of these are as bright or brighter than enhanced GFP when bound to TO1-Biotin. Furthermore, we show that the new Mangos can accurately image the subcellular localization of three small non-coding RNAs (5S, U6, and a box C/D scaRNA) in fixed and live mammalian cells. These new aptamers have many potential applications to study RNA function and dynamics both in vitro and in mammalian cells.
Date Issued
2018-02-13
Date Acceptance
2018-01-11
Citation
Nature Communications, 2018, 9 (1)
ISSN
2041-1723
Publisher
Nature Publishing Group
Journal / Book Title
Nature Communications
Volume
9
Issue
1
Copyright Statement
© The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
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Subjects
Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
GREEN FLUORESCENT PROTEIN
FLUOROPHORE-BINDING
CRYSTAL-STRUCTURE
STRUCTURAL BASIS
COMPLEX
SPECIFICITY
SELECTION
MIMICS
EXPRESSION
DYNAMICS
Aptamers, Nucleotide
Cell Line
Cells
Fluorescent Dyes
Humans
Microscopy, Fluorescence
RNA, Small Untranslated
Cells
Cell Line
Humans
Fluorescent Dyes
Microscopy, Fluorescence
Aptamers, Nucleotide
RNA, Small Untranslated
Publication Status
Published
Article Number
ARTN 656