Metabonomic Investigation of Single and Multiple Strain Trypanosoma brucei brucei Infections
Author(s)
Type
Journal Article
Abstract
Although co-infections are common and can have important epidemiologic and evolutionary consequences, studies exploring biochemical effects of multiple-strain infections remain scarce. We studied metabolic responses of NMRI mice to Trypanosoma brucei brucei single (STIB777AE-Green1 or STIB246BA-Red1) and co-infections using a 1H nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling strategy. All T. b. brucei infections caused an alteration in urinary biochemical composition by day 4 postinfection, characterized by increased concentrations of 2-oxoisocaproate, D-3-hydroxybutyrate, lactate, 4-hydroxyphenylacetate, phenylpyruvate, and 4-hydroxyphenylpyruvate, and decreased levels of hippurate. Although there were no marked differences in metabolic signatures observed in the mouse infected with a single or dual strain of T. b. brucei, there was a slower metabolic response in mice infected with T. b. brucei green strain compared with mice infected with either the red strain or both strains concurrently. Pyruvate, phenylpyruvate, and hippurate were correlated with parasitemia, which might be useful in monitoring responses to therapeutic interventions.
Date Issued
2011-01-01
Date Acceptance
2010-09-22
Citation
American Journal of Tropical Medicine and Hygiene, 2011, 84 (1), pp.91-98
ISSN
1476-1645
Publisher
American Society of Tropical Medicine and Hygiene
Start Page
91
End Page
98
Journal / Book Title
American Journal of Tropical Medicine and Hygiene
Volume
84
Issue
1
Copyright Statement
©The American Society of Tropical Medicine and Hygiene
This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Subjects
Science & Technology
Life Sciences & Biomedicine
Public, Environmental & Occupational Health
Tropical Medicine
PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH, SCI
TROPICAL MEDICINE
GLOBAL METABOLIC-RESPONSES
MICE
MARKERS
PLS
Publication Status
Published