OMIP-062: A 14-Color, 16-antibody panel for immunophenotyping human innate yymphoid, myeloid and T cells in small volumes of whole blood and pediatric airway samples
Author(s)
Type
Journal Article
Abstract
This 14‐color, 16‐antibody OMIP was designed for enumeration of leukocyte responses in pediatric samples, where sample volumes and cell numbers can be very low. Leukocytes identified by this panel include all major members of the innate lymphoid cell (ILC) family (ILC1s, ILC2s, and ILC3s), natural killer cells (NK cells), granulocytes (neutrophils and eosinophils), T‐cells (CD4+ and CD8+), mucosal‐associated invariant T cells (MAIT cells) and NKT‐like cells. The protocol was optimized using small volumes of peripheral blood and validated in airway samples obtained from children (< 2 years of age) admitted to a pediatric intensive care unit (PICU). Given this backdrop, this OMIP is widely applicable to clinical research using low volume or paucicellular samples, such as studies of innate and adaptive immune responses in infants and children, with potential clinical application in diagnostics and monitoring of patients by pediatricians.
Date Issued
2019-12-01
Date Acceptance
2019-09-19
Citation
Cytometry Part A, 2019, 95 (12), pp.1231-1235
ISSN
1552-4949
Publisher
Wiley
Start Page
1231
End Page
1235
Journal / Book Title
Cytometry Part A
Volume
95
Issue
12
Replaces
10044/1/74210
Copyright Statement
© 2019 The Authors. Cytometry Part A published by Wiley Periodicals, Inc. on behalf of International Society for Advancement of Cytometry. This is an open access article under the terms of the Creative Commons Attribution (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
Imperial College Healthcare NHS Trust- BRC Funding
Imperial College Healthcare NHS Trust- BRC Funding
Asthma UK
The Wellcome Trust
Asthma UK
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/31633878
Grant Number
RDA02
RDC04
Asthma UK Centre
109008/Z/15/A
AUK-BC-2015-01
Subjects
Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Cell Biology
Biochemistry & Molecular Biology
HETEROGENEITY
IMMUNITY
Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Cell Biology
Biochemistry & Molecular Biology
HETEROGENEITY
Antibodies
Blood Volume
Child
Color
Flow Cytometry
Fluorescent Antibody Technique
Humans
Immunity, Innate
Immunophenotyping
Infant
Lung
Lymphocytes
Myeloid Cells
T-Lymphocytes
Lung
Lymphocytes
T-Lymphocytes
Myeloid Cells
Humans
Antibodies
Fluorescent Antibody Technique
Flow Cytometry
Immunophenotyping
Blood Volume
Color
Child
Infant
Immunity, Innate
Immunology
0601 Biochemistry and Cell Biology
Publication Status
Published
Coverage Spatial
United States
Date Publish Online
2019-10-21