Changes in IgA-targeted microbiota following fecal transplantation for recurrent Clostridioides difficile infection
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
Secretory immunoglobulin A (IgA) interacts with intestinal microbiota and promotes mucosal homeostasis. IgA-bacteria interactions are altered during inflammatory diseases, but how these interactions are shaped by bacterial, host, and environmental factors remains unclear. In this study, we utilized IgA-SEQ to profile IgA-bound fecal bacteria in 48 recurrent Clostridioides difficile patients before and after successful fecal microbiota transplantation (FMT) to gain further insight. Prior to FMT, Escherichia coli was the most highly IgA-targeted taxon; following restoration of the microbiota by FMT, highly IgA-targeted taxa included multiple Firmicutes species. Post-FMT IgA-targeting was unaffected by the route of FMT delivery (colonoscopy versus capsule), suggesting that both methods lead to the establishment of healthy immune–bacterial interactions in the gut. Interestingly, IgA-targeting in FMT recipients closely resembled the IgA-targeting patterns of the donors, and fecal donor identity was significantly associated with IgA-targeting of the recipient microbiota. These data support the concept that intrinsic bacterial properties drive IgA recognition across genetically distinct human hosts. Together, this study suggests that IgA-bacterial interactions are reestablished in human FMT recipients to resemble that of the healthy fecal donor.
Date Issued
2021-01-01
Date Acceptance
2020-12-02
Citation
Gut Microbes, 2021, 13 (1), pp.1-12
ISSN
1949-0976
Publisher
Taylor and Francis
Start Page
1
End Page
12
Journal / Book Title
Gut Microbes
Volume
13
Issue
1
Copyright Statement
© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
License URL
Sponsor
Imperial College Healthcare NHS Trust- BRC Funding
Medical Research Council
Medical Research Council (MRC)
Grant Number
RDA02
MR/R00875/1
MR/R000875/1
Subjects
Clostridioides difficile
Immunoglobulin A
fecal microbiota transplant
microbiota
0605 Microbiology
Publication Status
Published
Date Publish Online
2020-12-31