Abnormal vaginal microbiome associated with vaginal mesh complications
File(s)
Author(s)
Type
Journal Article
Abstract
Aims To identify differences in the vaginal microbiomes of women after transvaginal mesh (TVM) surgery for pelvic organ prolapse with and without mesh‐associated complications. Methods Patients with complications were eligible as cases, patients without as controls. DNA was isolated and the V1‐2 region of the 16S ribosomal RNA gene was amplified and sequenced. Overall richness was quantified using Chao1. Overall diversity was expressed as Shannon diversity and screened for group differences using analysis of variance. Multivariate differences among groups were evaluated with functions from R . Results We recruited 14 patients after mesh exposure, 5 after contraction, and 21 as controls. The average number of operational taxonomic unit was 74.79 (SD ± 63.91) for controls, 57.13 (SD ± 58.74) after exposures, and 92.42 (SD ± 50.01) after contractions. Total 89.6% of bacteria in controls, 86.4% in previous exposures, and 81.3% in contractions were classified as either Firmicutes, Proteobacteria , or Actinobacteria (P < .001). Veillonella spp. was more abundant in patients after contraction (P = .045). The individual microbiomes varied, and we did not detect any significant differences in richness but a trend towards higher diversity with complications. Conclusions The presence of Veillonella spp. could be associated with mesh contraction. Our study did not identify vaginal microbiotic dysbiosis as a factor associated with exposure. Larger cohort studies would be needed to distinguish the vaginal microbiome of women predisposed to mesh‐related complications for targeted phenotyping of patients who could benefit from TVM surgery.
Date Issued
2019-08-11
Online Publication Date
2020-06-01T13:25:15Z
Date Acceptance
2019-07-22
ISSN
0733-2467
Publisher
Wiley
Start Page
2255
End Page
2263
Journal / Book Title
Neurourology and Urodynamics
Volume
38
Issue
8
Copyright Statement
© 2019 The Authors. Neurourology and Urodynamics published by Wiley Periodicals, Inc.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Identifier
https://onlinelibrary.wiley.com/doi/full/10.1002/nau.24129
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000480485900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
Subjects
Science & Technology
Life Sciences & Biomedicine
Urology & Nephrology
contraction
exposure
microbiome
transvaginal mesh
PELVIC ORGAN PROLAPSE
BACTERIAL VAGINOSIS
REPAIR
RISK
MANAGEMENT
SACROPEXY
SURGERY
contraction
exposure
microbiome
transvaginal mesh
Aged
Bacteria
DNA, Bacterial
Female
Humans
Microbiota
Middle Aged
Pelvic Organ Prolapse
Postoperative Complications
Surgical Mesh
Urologic Surgical Procedures
Vagina
Veillonella
Vagina
Humans
Bacteria
Veillonella
Postoperative Complications
DNA, Bacterial
Urologic Surgical Procedures
Surgical Mesh
Aged
Middle Aged
Female
Pelvic Organ Prolapse
Microbiota
Science & Technology
Life Sciences & Biomedicine
Urology & Nephrology
contraction
exposure
microbiome
transvaginal mesh
PELVIC ORGAN PROLAPSE
BACTERIAL VAGINOSIS
REPAIR
RISK
MANAGEMENT
SACROPEXY
SURGERY
Urology & Nephrology
1103 Clinical Sciences
1109 Neurosciences
Publication Status
Published
Country
Vienna, AUSTRIA
Date Publish Online
2019-08-11