A yeast-based tool for screening mammalian diacylglycerol acyltransferase inhibitors
Author(s)
Gajdos, P
Ledesma-Amaro, R
Nicaud, J-M
Rossignol, T
Type
Journal Article
Abstract
Dysregulation of lipid metabolism is associated with obesity and metabolic diseases but there is also increasing evidence of a relationship between lipid body excess and cancer. Lipid body synthesis requires diacylglycerol acyltransferases (DGATs) which catalyze the last step of triacylglycerol synthesis from diacylglycerol and acyl-coenzyme A. The DGATs and in particular DGAT2, are therefore considered potential therapeutic targets for the control of these pathologies. Here, the murine and the human DGAT2 were overexpressed in the oleaginous yeast Yarrowia lipolytica deleted for all DGAT activities, to evaluate the functionality of the enzymes in this heterologous host and DGAT activity inhibitors. This work provides evidence that mammalian DGATs expressed in Y. lipolytica are a useful tool for screening chemical libraries to identify potential inhibitors or activators of these enzymes of therapeutic interest.
Date Issued
2022-12-01
Date Acceptance
2022-10-21
ISSN
2045-8827
Publisher
Wiley
Start Page
1
End Page
10
Journal / Book Title
MicrobiologyOpen
Volume
11
Issue
6
Copyright Statement
© 2022 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Identifier
https://onlinelibrary.wiley.com/doi/10.1002/mbo3.1334
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Publication Status
Published
Article Number
ARTN e1334
Date Publish Online
2022-12-01