Bioconversion of cellulose into bisabolene using Ruminococcus flavefaciens and Rhodosporidium toruloides
File(s)
Published version
Author(s)
Type
Journal Article
Abstract
In this study, organic acids were demonstrated as a promising carbon source for bisabolene production by the non-conventional yeast, Rhodosporidium toruloides, at microscale with a maximum titre of 1055 ± 7 mg/L. A 125-fold scale-up of the optimal process, enhanced bisabolene titres 2.5-fold to 2606 mg/L. Implementation of a pH controlled organic acid feeding strategy at this scale lead to a further threefold improvement in bisabolene titre to 7758 mg/L, the highest reported microbial titre. Finally, a proof-of-concept sequential bioreactor approach was investigated. Firstly, the cellulolytic bacterium Ruminococcus flavefaciens was employed to ferment cellulose, yielding 4.2 g/L of organic acids. R. toruloides was subsequently cultivated in the resulting supernatant, producing 318 ± 22 mg/L of bisabolene. This highlights the feasibility of a sequential bioprocess for the bioconversion of cellulose, into biojet fuel candidates. Future work will focus on enhancing organic acid yields and the use of real lignocellulosic feedstocks to further enhance bisabolene production.
Date Issued
2023-01-01
Date Acceptance
2022-10-25
Citation
Bioresource Technology, 2023, 368
ISSN
0960-8524
Publisher
Elsevier
Journal / Book Title
Bioresource Technology
Volume
368
Copyright Statement
© 2022 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
License URL
Identifier
https://www.ncbi.nlm.nih.gov/pubmed/36347482
PII: S0960-8524(22)01549-8
Subjects
Biofuel
Bisabolene
Lignocellulose
Metabollic engineering
Microscale tools
Rhodosporidium toruloides
Rumen bacteria
Ruminococcus flavefaciens
Sequential bioreactors
Cellulose
Ruminococcus
Rhodotorula
Publication Status
Published
Coverage Spatial
England
Article Number
ARTN 128216
Date Publish Online
2022-11-05